Principles and Applications of Bioseparations
Fundamental scientific principles underlying the recovery, purification and formulation of biologics (biotherapuetics), especially proteins, are examined. Emphasis is placed on delineating the key chemical and physical properties of biomolecules that impact downstream processing and formulation development. Laboratories in the analytical and small-scale purification facility provide students with 'hands-on' exposure to key scientific principles and small scale unit operations. This is a half-semester course.
A student successfully completing BEC 330 should be able to:
- Describe the chemical and physical properties of biomacromolecules and how those properties influence the design of assays, downstream processes and product formulation.
- Explain the basic principles underlying common biopharmaceutical analytical, recovery, separation and purification methods such as spectroscopy, flocculation, precipitation, electrophoresis, membrane filtration, centrifugation, and chromatography.
- Understand the development of simple analytical models describing the above processes and utilize the models in the analysis of experimental data and process measurements.
Prerequisites & Requirements
Students must have passed CH 223 with a grade of C- or better or have Prof vZ's permission to take the course.
The following are recommended texts:
- “Physical Biochemistry: Principles and Applications” by D. Sheehan, Wiley & Sons, Second Edition 2009
- “Bioseparations Science and Engineering” by R. G. Harrison, P. W. Todd, S. R. Rudge & D. Petrides, Oxford University Press, 2002