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BEC/BME 483/583
Tissue Engineering Technologies

Course Description

In this half-semester laboratory module, students will:

  • Use advanced cell culture techniques to explore key topics in tissue engineering
  • Create complex skin equivalent tissue, including mesenchymal/extracellular matrix and epithelial layers; evaluate experimental biomaterials for potential to improve process
  • Investigate angiogenic capacity of engineered tissue using in vivo system
  • Fix, embed, section and stain engineered tissue constructs for structural analysis

Lecture Topics

  • Tissue engineering: state-of-the art, future directions
  • Biology of Tissues: epithelial/mesenchymal interactions
  • Skin as Model Organ: epidermal/dermal structure and differentiation
  • Angiogenesis: significance for tissue engineering
  • Theory and practice of: cell culture and co-culture; angiogenic assays; histology: fixation, paraffin embedding, sectioning and staining

Lab Topics

Week 1
Review cell culture techniques, lab safety, record keeping; Initiate dermal fibroblast cultures
Week 2
Cast dermal layer with cellular and acellular collagen components, plus experimental biomaterials; Initiate keratinocyte cultures
Week 3
Seed keratinocytes on dermal layer; manipulate medium and culture conditions to induce differentiation
Weeks 4-7
As skin equivalent tissues mature, test for ability to support angiogenesis using chick chorioallantoic membrane assay; Fix, embed, section and stain skin equivalents for analysis of tissue differentiation and effects of biomaterials

Prerequisites

  • BIT 466/566 — Students wishing to enroll without the prerequisite need to contact Susan Bernacki.