Tissue Engineering Technologies
In this half-semester laboratory module, students will:
- Use advanced cell culture techniques to explore key topics in tissue engineering
- Create complex skin equivalent tissue, including mesenchymal/extracellular matrix and epithelial layers; evaluate experimental biomaterials for potential to improve process
- Investigate angiogenic capacity of engineered tissue using in vivo system
- Fix, embed, section and stain engineered tissue constructs for structural analysis
- Tissue engineering: state-of-the art, future directions
- Biology of Tissues: epithelial/mesenchymal interactions
- Skin as Model Organ: epidermal/dermal structure and differentiation
- Angiogenesis: significance for tissue engineering
- Theory and practice of: cell culture and co-culture; angiogenic assays; histology: fixation, paraffin embedding, sectioning and staining
Review cell culture techniques, lab safety, record keeping; Cast dermal layer of skin with cellular and acellular collagen components, plus experimental biomaterials; Initiate keratinocyte cultures.
Seed keratinocytes on dermal layer; manipulate medium and culture conditions to induce differentiation.
Use Matrigel to perform endothelial cell tube angiogenesis assays; Design experiments to assess angiogenic potential of unknown agents.
Skin equivalent tissues matures. Test for ability of the tissue to support angiogenesis using a chick chorioallantoic membrane assay; Fix, paraffin embed, section and stain mature skin equivalent tissue for analysis of tissue differentiation and effects of biomaterials.
The normal pre-requisite, BIT 466/566, is not offered Fall 2023. Students with prior animal cell culture experience, for example BIT 410/510, other coursework, or laboratory research experience may enroll with permission of instructor. Please contact Dr. Bernacki at firstname.lastname@example.org.