Fundamentals of Mammalian Cell Line Development

  • June 18–20, 2024

Registration is closed.

Delivery methods

Course content

upstream course


This course can count toward your Professional Certificate in Biomanufacturing Science.

Why should you attend?

This course provides participants with fundamental knowledge of cutting-edge cloning strategies essential for mammalian cell line development, specifically geared towards protein overproduction with specific quality attributes. With a focus on the industrial-relevant Chinese hamster ovary (CHO DG44) cells as the expression model, attendees will delve into crucial aspects such as the selection of expression vectors, understanding host cell genetics and physiology, and implementing effective screening strategies to maximize production yields. Additionally, the course highlights various facets of cell culture techniques in small-scale disposable wave bags and glass bioreactors.

Attend and you will learn

  • Current CHO cell-based expression platforms (DG44 and GS systems)
  • Strategies for choosing the expression vector
  • CHO cell based genetics and physiology to maximize protein production
  • Mammalian cells transfection methods
  • Selection of the recombinant clones (dilution cloning)
  • Manual and automatic methods for screening the best producers
  • Gene editing tools (CRISPR, TALEN, ZNF, siRNA, etc.) for metabolic engineering
  • Best nutritional and environmental conditions for maximum recombinant protein production
  • Overview with hands-on experience in setting up and operating small-scale glass and wave bag disposable bioreactors for optimal mammalian cell growth and protein expression
  • Detection and analysis of the protein of interest (SDS-PAGE, Western blot, ELISA)
  • Cell banking (cryopreservation) and quality control (mycoplasma detection, etc.)

Who should attend

  • Professionals working in biopharmaceutical environments who are seeking background knowledge of mammalian cell line development
  • Professionals working in a research and development setting with new duties related to mammalian cell line development
  • Scientists with experience in other aspects of molecular biology planning to use mammalian cells for protein production
  • Those who hold an engineering degree with limited hands-on experience in molecular biology and genetic expression

Attend and you will receive

  • 22.5 hours of instruction (2.2 Continuing Education Units)
  • Notebook containing all course lecture and lab materials
  • Morning coffee and tea
  • Lunch
  • Afternoon beverage break
  • Certificate of completion


Driss Elhanafi, Ph.D.




All courses are offered on site at BTEC, other than online courses. The Golden LEAF BTEC building is located at 850 Oval Drive on NC State University's Centennial Campus.

If a course is full: Waitlist policy

If a course you want to take is already full, you are welcome to email and ask to be placed on a waitlist for that course. The waitlist is used to fill any seats that become available when individuals registered for the course are unable to attend. If one or more seats become available, individuals on the waitlist are contacted in the order in which they were added to the list.

In addition to ensuring popular courses remain full, waitlists provide a means of tracking interest and determining if additional sessions are necessary. BTEC makes every effort to schedule additional course offerings if sufficient people are wait-listed. If a new offering is scheduled, those on the waitlist are notified before registration opens to the public.

Placement on a waitlist does not commit individuals to attending a course. It simply ensures that they are given first opportunity to enroll if a seat becomes available in a full course and that they are among the first to know if an additional course offering is scheduled during the current year.

BTEC is unable to provide information about the number of those wait-listed for any given course or an individual's place on such a list.


BTEC requires payment by credit card at the time of registration. BTEC accepts payment from all major credit cards including American Express, Visa, and MasterCard.

After registration, a payment receipt is automatically emailed. If you do not receive the email, please check your spam folders.

Any additional questions prior to registration should be directed to

Discounts available

A 40% discount is available for NIIMBL members for the following courses:

Check out BTEC’s professional development certificate program for an additional way to save money on short course costs. Any additional discount questions prior to registration should be directed to Serena Hodge, Industry Training Coordinator, at

Cancellation policies

Cancellation by registrant

To cancel a registration and be eligible for a refund of course fees, you must notify BTEC by email to or fax to 919-513-1697. Fees are refunded according to the following schedule:

  • 100% refund – If notification is received at least 15 business days in advance of course start date
  • 75% refund – If notification is received 10–14 business days in advance of course start date
  • 50% refund – If notification is received 6–9 business days in advance of course start date
  • No refund will be issued if notice is received 5 or fewer business days in advance of course start

Refunds will take 60–90 days for processing. Refunds requested more than 90 days after payment will be issued as a check.

Substitutions may be made up to two business days prior to the course start date. Notify the BTEC office by email to or by fax at 919-513-1697. Please include the name and email contact for the replacement. Please note that BTEC is unable to honor substitutions for individuals taking discounted courses as part of the professional development certificate program.

Cancellation by BTEC

BTEC retains the right to cancel a professional development short course no less than 10 business days in advance of the scheduled course start date. Registrants will be notified by BTEC if a course is cancelled and will receive a full refund of registration fees paid. BTEC is not responsible for airfare penalties or other costs incurred due to cancellation.

Pre-course communication

Upon successful registration for a course, you will receive an automatically-generated email as well as another email from Melody Woodyard confirming your registration. If you do not receive these emails within two business days of registering for a course, please email to confirm your registration.

Once registered, confirmed course participants will receive a series of emails approximately two weeks before the scheduled course with detailed information regarding travel to BTEC, parking information, and a short pre-course questionnaire.