Applied Principles and Techniques of Depth Flow Filtration (DFF) and Tangential Flow Filtration (TFF) for BioPharm Downstream Purification

Registration options

October 22–25, 2024

*For Labs/workshop only please contact Melody Woodyard via email or phone: 919-513-8095.

Delivery methods

Course content

downstream course


This course can count toward your Professional Certificate in Biomanufacturing Science.

Why should you attend?

Through participation in this four-day comprehensive filtration course, you will learn the fundamental principles and optimum applied techniques for depth filtration (DFF) and tangential flow filtration (TFF) used today in biopharmaceutical downstream purification.

The one-day lecture is designed to help individuals gain a solid understanding of the parameters and factors that affect DFF and TFF membrane performance and product recovery for both upstream cell clarification and downstream UF/DF biopharmaceutical applications. Depth filtration (DFF) will cover polymeric membrane and matrix media technologies, and tangential flow filtration (TFF) will cover both flat sheet cassette and hollow fiber element configurations. New advancements will be covered.

During the three-day hands-on laboratory workshop, DFF and TFF application trials are performed using state-of-the-art benchtop systems. Two full-day sessions are dedicated to cell clarification—with DFF on day one and by TFF on day two. Day three focuses on UF/DF by TFF. Data developed during these trials will be summarized, discussed, and applied to real scale-up system problems and SOP. The principles and techniques presented in the prerequisite lecture course will be applied in this workshop.

Attend and you will learn

  • About filtration technologies and formats currently available for biopharmaceutical downstream purification
  • Current issues related to performance, scale-up, optimization, cleaning (CIP) and methods development through use of case studies
  • How to perform required initial membrane and element characterization
  • How to determine optimum operating conditions that meet trial objectives
  • How to apply the results to make informed, systematic decisions for scale-up and/or process improvements
  • How to perform process runs to include concentration and diafiltration with complete data acquisition and analysis
  • How to perform Clean-in-Place (CIP) to achieve maximum membrane recovery

Who should attend

The workshop is designed for engineers, scientists, managers, technical personnel and researchers who require an in-depth review of filtration principles and their application to processes in the areas of process development, manufacturing and quality assurance/validation.

Attend and you will receive

  • 29.75 hours of instruction (2.9 CEUs) – full 4-day course; 22.5 hours (2.2 CEUs) – 3-day laboratory workshop; 7.25 hours (0.7 CEUs) – 1-day lecture class only
  • Notebook containing all course lecture and lab materials
  • Morning coffee and tea
  • Lunch
  • Afternoon beverage break
  • Certificate of completion


Together with BTEC, this lecture and laboratory course is presented by John Rozembersky, president of Rozembersky Group, Inc., an independent biofiltration consulting firm he founded in 2005. Mr. Rozembersky’s hands-on experience and in-depth knowledge of filtration and purification span over 40 years with Millipore, Filtron, Pall, WaterSep, and currently ArteSyn Bio. He is an internationally recognized authority in TFF in the biopharmaceutical sector from benchtop development to large-scale manufacturing for both flat sheet (cassette) and hollow fiber formats. His focus the past several years has been addressing the challenges in single use applications and process systems by providing successful solutions to clients that result in higher performance, quality and yield of their products.


  • Full four-day course: $3,995
  • Three-day laboratory workshop: $3,195 (One-day lecture is a prerequisite.)
  • One-day lecture class only: $800


All courses are offered on site at BTEC, other than online courses. The Golden LEAF BTEC building is located at 850 Oval Drive on NC State University's Centennial Campus.

If a course is full: Waitlist policy

If a course you want to take is already full, you are welcome to email and ask to be placed on a waitlist for that course. The waitlist is used to fill any seats that become available when individuals registered for the course are unable to attend. If one or more seats become available, individuals on the waitlist are contacted in the order in which they were added to the list.

In addition to ensuring popular courses remain full, waitlists provide a means of tracking interest and determining if additional sessions are necessary. BTEC makes every effort to schedule additional course offerings if sufficient people are wait-listed. If a new offering is scheduled, those on the waitlist are notified before registration opens to the public.

Placement on a waitlist does not commit individuals to attending a course. It simply ensures that they are given first opportunity to enroll if a seat becomes available in a full course and that they are among the first to know if an additional course offering is scheduled during the current year.

BTEC is unable to provide information about the number of those wait-listed for any given course or an individual's place on such a list.


BTEC requires payment by credit card at the time of registration. BTEC accepts payment from all major credit cards including American Express, Visa, and MasterCard.

After registration, a payment receipt is automatically emailed. If you do not receive the email, please check your spam folders.

Any additional questions prior to registration should be directed to

Discounts available

A 40% discount is available for NIIMBL members for the following courses:

Check out BTEC’s professional development certificate program for an additional way to save money on short course costs. Any additional discount questions prior to registration should be directed to Serena Hodge, Industry Training Coordinator, at

Cancellation policies

Cancellation by registrant

To cancel a registration and be eligible for a refund of course fees, you must notify BTEC by email to or fax to 919-513-1697. Fees are refunded according to the following schedule:

  • 100% refund – If notification is received at least 15 business days in advance of course start date
  • 75% refund – If notification is received 10–14 business days in advance of course start date
  • 50% refund – If notification is received 6–9 business days in advance of course start date
  • No refund will be issued if notice is received 5 or fewer business days in advance of course start

Refunds will take 60–90 days for processing. Refunds requested more than 90 days after payment will be issued as a check.

Substitutions may be made up to two business days prior to the course start date. Notify the BTEC office by email to or by fax at 919-513-1697. Please include the name and email contact for the replacement. Please note that BTEC is unable to honor substitutions for individuals taking discounted courses as part of the professional development certificate program.

Cancellation by BTEC

BTEC retains the right to cancel a professional development short course no less than 10 business days in advance of the scheduled course start date. Registrants will be notified by BTEC if a course is cancelled and will receive a full refund of registration fees paid. BTEC is not responsible for airfare penalties or other costs incurred due to cancellation.

Pre-course communication

Upon successful registration for a course, you will receive an automatically-generated email as well as another email from Melody Woodyard confirming your registration. If you do not receive these emails within two business days of registering for a course, please email to confirm your registration.

Once registered, confirmed course participants will receive a series of emails approximately two weeks before the scheduled course with detailed information regarding travel to BTEC, parking information, and a short pre-course questionnaire.