Sartorius Fermentation Roadshow

Join Sartorius on Friday, Oct. 23 for a one-day roadshow seminar and live fermentation. 

Equipment on Display

  • ambr15
  • Biostat B 2 Liter
  • Biostat RMII 20 Liter
  • Biostat STR 50 Liter
  • BioPAT Spectro NIR Multiparameter Analyzer
  • BioPAT Trace Glucose/Lactate Analyzer
  • BioPAT Viamass Viable Cell Density
  • BioPAT Xgas Offgas Analyzer

Poster Presentation (10:00-12:00)
NIR-Spectroscopy for Bioprocess Monitoring

D. Ornek1, J. Friel1, R. Anderson1, D. Haskew1, C. Yon1, and D. Kopec2

1 FUJIFILM Diosynth Biotechnologies U.S.A., Inc.
2 Sartorius Stedim North America.

The BioPAT® Spectro Near-infrared spectroscopy (NIR) free-beam technology offers an effective PAT tool for QbD drug development. It is a nondestructive analytical technique that has been used for real-time, in-situ monitoring of substrates, products, and biomass in microbial and cell cultures processes. It can provide a significant opportunity for bioprocess understanding, development, optimization, improvement, and cost savings during drug development. The objectives of this study were to evaluate the potential of NIR-Spectroscopy to simultaneously predict concentrations of critical analytes in yeast fermentation and to create batch trajectories based on NIR spectra. Measurements were made in situ, by attaching the BioPAT® Spectro NIR probe directly to a 10L steam-in-place fermenter providing direct contact with the fermentation culture media. Extended least Squares (XLS) regression was used to develop calibration models between spectral and off-line analytical data to quantitatively predict the concentrations of analytes. Batch trajectories were created using Batch Evolution Model via NIR spectra, to qualitatively determine process variability. The models created show this technology has excellent potential to deliver fast and robust prediction of fermentation media analyte trends.

LIVE Fermentation (12:00-2:00)

NIR BioPAT® Spectro Presentation by Dan Kopec, field marketing manager. In this real-time multi-parameter monitoring of E. coli process, participants will be able to view a live demonstration of the BioPAT® Spectro on a 30L stainless bioreactor, with display of multiple simultaneous CPP’s measured every 15 seconds in situ.

Review the event flyer for more information, and email if you plan to attend.