Fundamentals of Mammalian Cell Line Development
This course provides participants with fundamental knowledge of cutting-edge cloning strategies essential for mammalian cell line development, specifically geared towards protein overproduction with specific quality attributes. With a focus on the industrial-relevant Chinese hamster ovary (CHO DG44) cells as the expression model, attendees will delve into crucial aspects such as the selection of expression vectors, understanding host cell genetics and physiology, and implementing effective screening strategies to maximize production yields. Additionally, the course highlights various facets of cell culture techniques in small-scale disposable wave bags and glass bioreactors.
| Dates | Course | Cost | Registration |
|---|---|---|---|
| May 12-13, 2026 | Artificial Intelligence in FDA-Regulated Manufacturing: From Foundations to Implementation | $1,500 | Register |
| May 20–21, 2026 | Hands-On Essentials of Automation for Biomanufacturing | $2,050 | Register |
| May 27–29, 2026 | Preparative Chromatography Column Packing: Lab to Process Scale | $2,500 | Register |
| June 3–4, 2026 | Applied Cleaning Validation Practices: A STERIS Master Class | $2,000 | Register |
| June 9–10, 2026 | Introduction to Biopharmaceutical Lyophilization | $1,975 | Register |
| June 11, 2026 | Essentials of Spray Drying and Powder Analysis | $995 | Register |
| June 16–18, 2026 | Fundamentals of Mammalian Cell Line Development | $3,100 | Register |
| June 23–25, 2026 | Biopharmaceutical Assay Essentials | $3,100 | Register |
| June 23–25, 2026 | Fermentation Engineering | $3,100 | Register |
| June 30–July 2, 2026 | Hands-On Principles of mRNA Technology | $2,500 | Register |
| July 8–10, 2026 | Downstream Biopharmaceutical Processes: Fundamentals and Design | $3,100 | Register |
| July 14–16, 2026 | AAV Quality Attributes: Theory and Practice | $2,975 | Register |
| July 14–17, 2026 | Hands-On cGMP Biomanufacturing Operations | $4,200 | Register |
| July 28–31, 2026 | Hands-On Biomanufacturing of Vectors for Gene Therapy | $4,500 | Register |
| Aug 6, 2026 | Design of Experiments for Biomanufacturing | $500* | Register |
| Sept 29–Oct 1, 2026 | Cell Culture Engineering | $4,200 | Register |
| Oct 13–16, 2026 | Applied Principles and Techniques of Depth Flow Filtration (DFF) and Tangential Flow Filtration (TFF) for BioPharm Purification | $3,995 | Register |
| Nov 10–12, 2026 | PAT for Real-time Culture Monitoring and Closed-Loop Process Control | $2,975 | Register |
| TBD | Microbial Contamination Control in Bioprocessing Operations | $2,975 |
Register by June 1
Attend and you will learn
- Current CHO cell-based expression platforms (DG44 and GS systems)
- Strategies for choosing the expression vector
- CHO cell based genetics and physiology to maximize protein production
- Mammalian cells transfection methods
- Selection of the recombinant clones (dilution cloning)
- Manual and automatic methods for screening the best producers
- Gene editing tools (CRISPR, TALEN, ZNF, siRNA, etc.) for metabolic engineering
- Best nutritional and environmental conditions for maximum recombinant protein production
- Overview with hands-on experience in setting up and operating small-scale glass and wave bag disposable bioreactors for optimal mammalian cell growth and protein expression
- Detection and analysis of the protein of interest (SDS-PAGE, Western blot, ELISA)
- Cell banking (cryopreservation) and quality control (mycoplasma detection, etc.)
Delivery methods
40% Lecture
10% Discussion/group activity
50% Hands-on lab experience
Course content
25% Fundamentals and concepts
50% Industry applications
25% Emerging technologies
22.5 hours of instruction
Who should attend
- Professionals working in biopharmaceutical environments who are seeking background knowledge of mammalian cell line development
- Professionals working in a research and development setting with new duties related to mammalian cell line development
- Scientists with experience in other aspects of molecular biology planning to use mammalian cells for protein production
- Those who hold an engineering degree with limited hands-on experience in molecular biology and genetic expression