PAT for Real-time Culture Monitoring and Closed-Loop Process Control

Register for this course by selecting one of the dates above.

Delivery methods

Course content

upstream course

Analytical or Automation

This course can count toward your Professional Certificate in Biomanufacturing Science.

Why should you attend?

In this three-day, hands-on course, you will gain familiarity with and applied insight into the functionality of control equipment and instrumentation, and how to integrate Process Analytical Technology (PAT) into a model yeast fermentation for more advanced in-line monitoring and process control. Participants will work with traditional sensors, a PAT spectral sensor, and multivariate data analysis (MVDA) software, to monitor, model, and control a model process.

This course will give professionals with experience in fermentation an in-depth introduction to process instrumentation and its integration into a distributed control system. Professionals will learn how process information is communicated and used for different engineering controls, including PID control, split-range control, and cascade control within the DeltaV distributed control system. A Raman spectral sensor and Partial Least Squares (PLS) model will be deployed for process monitoring and control to familiarize participants with implementing PAT in an upstream process. Participants will gain hands-on experience with data analytics software to build a data model for monitoring in real-time to enable better control of process parameters such as substrate concentration, and allow tighter control of the culture process. Coming out of this course professionals will have the knowledge of how to deploy a PAT system for an upstream process.

Attend and you will learn

  • To design and set up basic process monitoring, feedback, and control loop systems
  • To use commercial MVDA software to model and monitor process parameters in a yeast culture
  • To utilize a PAT sensor and PLS modeling for process monitoring and feedback control

Who should attend

This course is intended for scientists and engineers familiar with biomanufacturing processes, and with some experience in fermentation, who are interested in gaining insight into how the control equipment functions and how PAT sensors are employed to monitor a process. This course is appropriate for upstream process engineers, manufacturing scientists, process developers, and integrators who are interested in learning more about using analytics and predictive modeling to monitor and control the process. Participants do not need advanced knowledge of process automation or the use of analytics to build models for this course.

Attend and you will receive

  • 22 hours of instruction
  • Notebook containing all course lecture and lab materials
  • Morning coffee and tea
  • Lunch
  • Afternoon beverage break
  • Certificate of completion





All courses are offered on site at BTEC, other than online courses. The Golden LEAF BTEC building is located at 850 Oval Drive on NC State University's Centennial Campus.

If a course is full: Waitlist policy

If a course you want to take is already full, you are welcome to email and ask to be placed on a waitlist for that course. The waitlist is used to fill any seats that become available when individuals registered for the course are unable to attend. If one or more seats become available, individuals on the waitlist are contacted in the order in which they were added to the list.

In addition to ensuring popular courses remain full, waitlists provide a means of tracking interest and determining if additional sessions are necessary. BTEC makes every effort to schedule additional course offerings if sufficient people are wait-listed. If a new offering is scheduled, those on the waitlist are notified before registration opens to the public.

Placement on a waitlist does not commit individuals to attending a course. It simply ensures that they are given first opportunity to enroll if a seat becomes available in a full course and that they are among the first to know if an additional course offering is scheduled during the current year.

BTEC is unable to provide information about the number of those wait-listed for any given course or an individual's place on such a list.


BTEC requires payment by credit card at the time of registration. BTEC accepts payment from all major credit cards including American Express, Visa, and MasterCard.

After registration, a payment receipt is automatically emailed. If you do not receive the email, please check your spam folders.

Any additional questions prior to registration should be directed to

Discounts available

A 20% discount is available for NIIMBL members for the following courses:

Check out BTEC’s professional development certificate program for an additional way to save money on short course costs. Any additional discount questions prior to registration should be directed to Serena Hodge, Industry Training Coordinator, at

Cancellation policies

Cancellation by registrant

To cancel a registration and be eligible for a refund of course fees, you must notify BTEC by email to or fax to 919-513-1697. Fees are refunded according to the following schedule:

  • 100% refund – If notification is received at least 15 business days in advance of course start date
  • 75% refund – If notification is received 10–14 business days in advance of course start date
  • 50% refund – If notification is received 6–9 business days in advance of course start date
  • No refund will be issued if notice is received 5 or fewer business days in advance of course start

Refunds will take 60–90 days for processing. Refunds requested more than 90 days after payment will be issued as a check.

Substitutions may be made up to two business days prior to the course start date. Notify the BTEC office by email to or by fax at 919-513-1697. Please include the name and email contact for the replacement. Please note that BTEC is unable to honor substitutions for individuals taking discounted courses as part of the professional development certificate program.

Cancellation by BTEC

BTEC retains the right to cancel a professional development short course no less than 10 business days in advance of the scheduled course start date. Registrants will be notified by BTEC if a course is cancelled and will receive a full refund of registration fees paid. BTEC is not responsible for airfare penalties or other costs incurred due to cancellation.

Pre-course communication

Upon successful registration for a course, you will receive an automatically-generated email as well as another email from Melody Woodyard confirming your registration. If you do not receive these emails within two business days of registering for a course, please email to confirm your registration.

Once registered, confirmed course participants will receive a series of emails approximately two weeks before the scheduled course with detailed information regarding travel to BTEC, parking information, and a short pre-course questionnaire.