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Fundamentals of Mammalian Cell Line Development

July 25–27, 2017

Register through our online system by selecting the link above.

Delivery methods

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bar graph of hands-on lab experience delivery 50% Hands-on lab experience

Course content

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downstream course

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This course can count toward your Professional Certificate in Biomanufacturing Science.

Why should you attend?

Individuals attending this course gain a fundamental knowledge of the latest, most advanced cloning strategies vital to mammalian cell line development for protein production. Using Chinese hamster ovary (CHO) cells as the expression model, this course emphasizes the choice of expression vector, host cell genetics and physiology, and screening strategies to assure the highest production yields. Practical hands-on laboratory sessions include DNA cloning, cell transfection, selection/screening for best producer, and the expression/analysis of the protein of interest.

Attend and you will learn

  • Current CHO cell-based expression platforms
  • Strategies for choosing the expression vector  
  • CHO cell genetics to maximize protein production
  • Transfection of mammalian cells  
  • Selection of the recombinant clones
  • Methods for screening the best producers 
  • Best environmental conditions for protein production
  • Detection and analysis of the protein of interest
  • Cell line storage with cryopreservation

Who should attend

  • Professionals working in biopharmaceutical environments who are seeking background knowledge of mammalian cell line development
  • Professionals working in a research and development setting with new duties related to mammalian cell line development
  • Scientists with experience in other aspects of molecular biology planning to use mammalian cells for protein production
  • Those who hold an engineering degree with limited hands-on experience in molecular biology and genetic expression

Attend and you will receive

  • 22.5 hours of instruction (2.2 Continuing Education Units)
  • Morning coffee
  • Lunch
  • Afternoon beverage break
  • Certificate of attendance

Instructors

Driss Elhanafi, Ph.D.

Fee

$1,845


Location

All courses are offered on site at BTEC, other than online courses. The Golden LEAF BTEC building is located at 850 Oval Drive on NC State University's Centennial Campus.

Payment

BTEC accepts payment from all major credit cards including American Express, Visa, and MasterCard. If you wish to pay by company check, please email melody_woodyard@ncsu.edu for additional information immediately after registering.

Discounts available

A 20% discount is available to:

A 30% discount is available to faculty/staff working in academic environments.

Check out BTEC’s professional development certificate program for an additional way to save money on short course costs.

Short course cancellations

Cancellation by registrant

To cancel a registration and be eligible for a refund of course fees, you must notify BTEC by e-mail to Melody_Woodyard@ncsu.edu or fax to 919-513-1697. Fees are refunded according to the following schedule:

  • 100% refund – If notification is received at least 15 business days in advance of course start date
  • 75% refund – If notification is received 10–14 business days in advance of course start date
  • 50% refund – If notification is received 6–9 business days in advance of course start date
  • No refund will be issued if notice is received 5 or fewer business days in advance of course start

Substitutions may be made up to two business days prior to the course start date. Notify the BTEC office by fax at 919-513-1697 or by e-mail to Melody_Woodyard@ncsu.edu. Please include the name and email contact for the replacement. Please note that BTEC is unable to honor substitutions for individuals taking discounted courses as part of the professional development certificate program.

Cancellation by BTEC

BTEC retains the right to cancel a professional development short course no less than 10 business days in advance of the scheduled course start date. Registrants will be notified by BTEC if a course is cancelled and will receive a full refund of registration fees paid. BTEC is not responsible for airfare penalties or other costs incurred due to cancellation.

Pre-Course Communication

Registered course participants will receive an email two weeks before the scheduled course with detailed information regarding travel to BTEC, parking information, and a short pre-course questionnaire.