BEC 488/588/CHE 488
Animal Cell Culture Engineering
This course will provide students with practical experience with key cell culture techniques and processes used by the biomanufacturing industry, with a strong focus on single-use bioreactors. Specific areas of focus include: cell banking, gene therapy vector production, Design of Experiment (DOE), and operation of bioreactors in various modes: batch, fed-batch and perfusion. This is a half-semester course offered in the spring.
- Introduction to cell culture and cell banking.
- Gene therapy vector production.
- Design of Experiments (DOE).
- Strategies for enhancing protein production: Gene editing tools and metabolic engineering.
- Media, bioreactor design, modes of operation.
- Scale-up parameters and critical process parameters.
- Scale up and banking of HEK293 cells grown in a 10-stack.
- Plaque purification of a Baculovirus stock.
- Design of Experiment (DOE): Optimization of transient transfection into HEK293 cells to produce AAV2-GFP.
- Production of AAV9-GFP (baculovirus): Scale-up and infection of insect cells in a 50-L single use bioreactor (SUB).
- Production of a monoclonal antibody (mAb) using the ambr250 Modular bioreactors.
- Production of a monoclonal antibody (mAb) using Alternating Tangential Flow (ATF) perfusion.
BEC 463, BEC 441 or BIT 466 or industry experience