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BTEC and UNC Core Facility join forces to offer unified contract services

Published Oct. 27, 2015

BTEC and the UNC Protein Expression and Purification Core Facility will collaborate to offer integrated development services to university researchers and emerging companies. The partnership will provide “one-stop shopping” for clients looking for cell lines that express their proteins and bioprocesses that produce commercially viable yields, at the right purities, and are capable of meeting all regulatory expectations, such as Good Manufacturing Practice (GMP).

The UNC Protein Expression and Purification (PEP) Core Facility, under the direction of Michael J. Miley, Ph.D., specializes in the production of pure, functional proteins for structural, biophysical, and biochemical studies. The unit currently utilizes E. coli, insect cell (baculovirus mediated), and mammalian cell expression systems. Typically, users provide a sequence-verified expression vector containing the gene(s) of interest. Alternatively, PEP staff can undertake expression vector construction if requested.

BTEC has been providing contract services to academia and industry since 2009. Upstream scientists have experience developing optimized cell culture processes and scaling them up to one or more of BTEC’s 20L, 50L or 250L single-use disposable bioreactors. Downstream scientists can develop the processes to remove cells and then purify the target protein via chromatography and ultrafiltration.

For more information about services that can be provided, contact Rick Lawless, Associate Director for Strategic Programs at BTEC, at rick_lawless@ncsu.edu.