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Recent publications by BTEC faculty and staff

Each year, faculty and instructional staff affiliated with BTEC publish numerous articles in both peer-reviewed journals and industry publications.

2023

Brown, P., Chen, Y., Ivanova, M., Leekitcharoenphon, P., Parsons, C., Niedermeyer, J., Gould, N., Strules, J., Mesa-Cruz, J. B., Kelly, M. J., Hooker, M. J., Chamberlain, M. J., Olfenbuttel, C., DePerno, C., Elhanafi, D., & Kathariou, S. (2023). Draft genome sequences of 158 listeria monocytogenes strains isolated from black bears (ursus americanus) in the united states. Microbiology Resource Announcements, 12(7), e0024823-e0024823. https://doi.org/10.1128/mra.00248-23

Brown, P., Kanenaka, R., Chen, Y., Ivanova, M., Leekitcharoenphon, P., Elhanafi, D., & Kathariou, S. (2023). Draft genome sequences of closely related listeria monocytogenes lineage III strains isolated from a food processing environment and a case of human listeriosis. Microbiology Resource Announcements, 12(6), e0025023-e0025023. https://doi.org/10.1128/mra.00250-23

Brown, P., Lee, S., Elhanafi, D., Tham, W., Danielsson-Tham, M., Lopez-Valladares, G., Chen, Y., Ivanova, M., Leekitcharoenphon, P., & Kathariou, S. (2023). Investigation of a listeria monocytogenes chromosomal immigration control region reveals diverse restriction modification systems with complete sequence type conservation. Microorganisms (Basel), 11(3), 699. https://doi.org/10.3390/microorganisms11030699

Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback‐Clarke, P., Smith, W., Moore, B., Kilgore, R., Cummings, C., Pancorbo, J., Gilleskie, G., Daniele, M. A., & Menegatti, S. (2023). Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates. Biotechnology and Bioengineering, https://doi.org/10.1002/bit.28495

Fan, J., Sripada, S. A., Pham, D. N., Linova, M. Y., Woodley, J. M., Menegatti, S., Boi, C., & Carbonell, R. G. (2023). Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane. Separation and Purification Technology, 317, 123920. https://doi.org/10.1016/j.seppur.2023.123920

Karakis, V., Jabeen, M., Britt, J. W., Cordiner, A., Mischler, A., Li, F., San Miguel, A., & Rao, B. M. (2023). Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions. The Journal of Biological Chemistry, 299(5), 104650-104650. https://doi.org/10.1016/j.jbc.2023.10465

Kilgore, R., Chu, W., Bhandari, D., Fischler, D., Carbonell, R. G., Crapanzano, M., & Menegatti, S. (2023). Development of peptide affinity ligands for the purification of polyclonal and monoclonal fabs from recombinant fluids. Journal of Chromatography A, 1687, 463701. https://doi.org/10.1016/j.chroma.2022.463701

Kilgour, K. M., Turner, B. L., Daniele, M., & Menegatti, S. Dual-affinity ratiometric quenching (DARQ) assay for the quantification of therapeutic antibodies in CHO‑S cell culture fluids. Accepted for publication June 9, 2023, by Analytical Chemistry (Washington).

Lavoie, J., Fan, J., Pourdeyhimi, B., Boi, C., & Carbonell, R. G. (2023). Advances in high-throughput, high-capacity nonwoven membranes for chromatography in downstream processing: A review. Biotechnology and Bioengineering, https://doi.org/10.1002/bit.28457

Mukherjee, R. P., Yow, G., Sarakbi, S., Menegatti, S., Gurgel, P. V., Carbonell, R. G., & Bobay, B. G. (2023). Integrated in silico and experimental discovery of trimeric peptide ligands targeting butyrylcholinesterase. Computational Biology and Chemistry, 102, 107797-107797. https://doi.org/10.1016/j.compbiolchem.2022.107797

Oh, Y. H., Becker, M. L., Mendola, K. M., Choe, L. H., Min, L., Lee, K. H., Yigzaw, Y., Seay, A., Bill, J., Li, X., Roush, D. J., Cramer, S. M., Menegatti, S., & Lenhoff, A. M. (2023). Characterization and implications of host‐cell protein aggregates in biopharmaceutical processing. Biotechnology and Bioengineering, 120(4), 1068-1080. https://doi.org/10.1002/bit.28325

Oh, Y.H., Mendola, K. M., Choe, L. H., Lavoie, A. J., Lavoie, A. R., Williams, T. I., Lee, K. H., Yigzaw, Y., Seay, A., Li, X., Roush, D. J., Cramer, S. M., Menegatti, S., & Lenoff, A. M. (n.d.). Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing. Accepted for publication June 5, 2023, by Biotechnology and Bioengineering.

Overton, L., Boi, C., Shastry, S., Smith-Moore, C., Balchunas, J., Sambadan, D., & Gilleskie, G. (2023). Development and Delivery of a Hands-On Short Course in Adeno-Associated Virus Manufacturing to Support Growing Workforce Needs in Gene Therapy. Human Gene Therapy, 34(7-8), 259–272. https://doi.org/http://doi.org/10.1089/hum.2022.235

Prodromou, R., Moore, B. D., Chu, W., Deal, H., San Miguel, A., Brown, A. C., Daniele, M. A., Pozdin, V. A., & Menegatti, S. (2023). Molecular engineering of cyclic Azobenzene‐Peptide hybrid ligands for the purification of human blood factor VIII via Photo‐Affinity chromatography. Advanced Functional Materials, 33(14), n/a. https://doi.org/10.1002/adfm.202213881

Sadat, A., Farag, A. M. M., Elhanafi, D., Awad, A., Elmahallawy, E. K., Alsowayeh, N., El-Khadragy, M. F., & Elshopakey, G. E. (2023). Immunological and oxidative biomarkers in bovine serum from healthy, clinical, and sub-clinical mastitis caused by escherichia coli and staphylococcus aureus infection. Animals (Basel), 13(5), 892. https://doi.org/10.3390/ani13050892

Stevnsborg, M., Selle, K., Barton, R., Prado-Rubio, O. A., Gargalo, C., Gernaey, K. V., Gilleskie, G., & Huusom, J. K. (2023). Digital Twins in Pilot Scale Fermentation: Non-Linear State Estimation for Improving Induction Timing. In Computer Aided Chemical Engineering – Vol. 52, 33rd European Symposium on Computer Aided Process Engineering (pp. 2637–2642). Elsevier. https://doi.org/10.1016/B978-0-443-15274-0.50419-4.

Turner, B. L., Twiddy, J., Wilkins, M. D., Ramesh, S., Kilgour, K. M., Domingos, E., Nasrallah, O., Menegatti, S., & Daniele, M. A. (2023). Biodegradable elastomeric circuit boards from citric acid-based polyesters. Npj Flexible Electronics, 7(1), 25-14. https://doi.org/10.1038/s41528-023-00258-z

Twiddy, J., Sharkey, C., Aroche, A. F., Peterson, K., Menegatti, S., & Daniele, M. A. (n.d.). Towards electrochemical control of Ph for regeneration of biosensors. Accepted for publication June 2, 2023, by IEEE BioSensors.

Wang, J., Hosseini M., Barbieri, E., Shastry, S., Chu, W. Menegatti. S., & Daniele, M, “Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy”, accepted for publication June 2, 2023, by IEEE BioSensors.

2022

Bacon, Kaitlyn, Stefano Menegatti, and Balaji M. Rao. 2022. "Discovery of Cyclic PeptideCyclic Peptides Binders from Chemically Constrained Yeast Display Libraries." In Yeast Surface Display, 387-415. New York, NY: Springer US.

Boi, Cristiana, Andrea Malavasi, Ruben G. Carbonell, and Gary Gilleskie. 2020. "A Direct Comparison between Membrane Adsorber and Packed Column Chromatography Performance." Journal of Chromatography A 1612: 460629. DOI: 10.1016/j.chroma.2019.460629

Chu, Wenning, Raphael Prodromou, Brandyn Moore, Driss Elhanafi, Ryan Kilgore, Shriarjun Shastry, and Stefano Menegatti. 2022. "Development of Peptide Ligands for the Purification of α-1 Antitrypsin from Cell Culture Fluids." Journal of Chromatography A 1679: 463363. https://doi.org/10.1016/j.chroma.2022.463363

Barbieri, E., Cutright, C. C., Ramesh, S., Khan, Saad A., Efimenko, K., Genzer, J., & Menegatti, S. (2022). Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers. Advanced Materials Interfaces, 9(33). https://doi.org/https://doi.org/10.1002/admi.202270183

Castillo, G. A., Dickey, M. D., Gorman, C. B., Genzer, J., & Efimenko, K. (2022). Deposition of silicate coatings on poly(ethylene terephthalate) for improved scratch and solvent resistance. Journal of Applied Polymer Science, 139(11), 51800-n/a. https://doi.org/10.1002/app.51800

Chu, W., Prodromou, R., Moore, B., Elhanafi, D., Kilgore, R., Shastry, S., & Menegatti, S. (2022). Development of peptide ligands for the purification of α-1 antitrypsin from cell culture fluids. Journal of Chromatography A, 1679, 463363. https://doi.org/10.1016/j.chroma.2022.463363

Conner, C. G., McAndrew, J., Menegatti, S., & Velev, O. D. (2022). An accelerated antibody aggregation test based on time sequenced dynamic light scattering. Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 653, 129833. https://doi.org/10.1016/j.colsurfa.2022.129833

Davis B.A., Bennett J.A., Genzer J., Efimenko K., Abolhasani M. (2022). Intensified Hydrogenation in Flow Using a Poly (β-cyclodextrin) Network-Supported Catalyst. ACS Sustainable Chemistry & Engineering, 10(48), 15987-15998.

Fahr, S., Peña-Benavides, S. A., Thiel, L., Sengoba, C., Karacasulu, K., Ihling, N., Sosa-Hernández, J. E., Gilleskie, G., Woodley, J. M., Parra-Saldivar, R., Mansouri, S. S., & Roh, K. (2022). Mobile on demand COVID-19 vaccine production units for developing countries. Industrial & Engineering Chemistry Research, 61(35), 13191-13204. https://doi.org/10.1021/acs.iecr.2c01217

Fan, J., Barbieri, E., Shastry, S., Menegatti, S., Boi, C., & Carbonell, R. G. (2022). Purification of adeno-associated virus (AAV) serotype 2 from spodoptera frugiperda (Sf9) lysate by chromatographic nonwoven membranes. Membranes (Basel), 12(10), 944. https://doi.org/10.3390/membranes12100944

Hetzler, Z., Wang, Y., Krafft, D., Jamalzadegan, S., Overton, L., Kudenov , M. W., Ligler, F. S., & Wei, Q. (2022). Flexible sensor patch for continuous carbon dioxide monitoring. Frontiers in Chemistry, 10. https://doi.org/10.3389/fchem.2022.983523

Kedia, S. B., Baker, J. C., Carbonell, R. G., Lee, K. H., Roberts, C. J., Erickson, J., Schiel, J. E., Rogers, K., Schaefer, G., & Pluschkell, S. (2022). Biomanufacturing readiness levels [BRL]—A shared vocabulary for biopharmaceutical technology development and commercialization. Biotechnology and Bioengineering, 119(12), 3526-3536. https://doi.org/10.1002/bit.28227

Lee, B.H., Aroche, A. F., Menegatti, S., & Daniele, M. A. Toward an Aptasensor for Monitoring of Tacrolimus, 2022 IEEE Sensors, Dallas, TX, USA, 2022, pp. 1-4, doi: 10.1109/SENSORS52175.2022.996726.

Machikiti, Z., Pourdeyhimi, B., Genzer, J., & Efimenko, K. (2022). Controlling PA6/PET adhesion to facilitate interfacial fracture. European Polymer Journal, 171, 111196. https://doi.org/10.1016/j.eurpolymj.2022.111196

McArthur, N., Cruz-Teran, C., Thatavarty, A., Reeves, G. T., & Rao, B. M. (2022). Experimental and analytical framework for “Mix-and-read” assays based on split luciferase. ACS Omega, 7(28), 24551-24560. https://doi.org/10.1021/acsomega.2c02319

National Academies of Sciences, Engineering, and Medicine. 2022. Globally resilient supply chains for seasonal and pandemic influenza vaccines. Washington, DC: The National Academies Press. https://doi.org/10.17226/26285. (J. Pancorbo, Committee on Addressing Issues of Vaccine Distribution and Supply Chains to Advance Pandemic and Seasonal Influenza Preparedness and Response.)

Ramesh, S., Davis, J., Roros, A., Zhou, C., He, N., Gao, W., Khan, S., Genzer, J., & Menegatti, S. (2022). Nonwoven membranes with infrared light-controlled permeability. ACS Applied Materials & Interfaces, 14(37), 42558-42567. https://doi.org/10.1021/acsami.2c13280

Rittschof D., Orihuela B., Genzer J., Efimenko K. (2022). PDMS networks meet barnacles: a complex and often toxic relationship. Biofouling, doi: 10.1080/08927014.2022.2145471.

Rutter C, Pancorbo Jennifer. 2020. A Lab-Scale Fermentation Course with an Emphasis on Recombinant Protein Production. Chemical Engineering Education 54:4 202–212. https://doi.org/10.18260/2-1-370.660-116386

Sripada, S. A., Chu, W., Williams, T. I., Teten, M. A., Mosley, B. J., Carbonell, R. G., Lenhoff, A. M., Cramer, S. M., Bill, J., Yigzaw, Y., Roush, D. J., & Menegatti, S. (2022). Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents. Biotechnology and Bioengineering, 119(7), 1873-1889. https://doi.org/10.1002/bit.28096

2021

Gilleskie, Gary, Rutter, Charles and McCuen, Becky. Biopharmaceutical Manufacturing: Principles, Processes, and Practices. Berlin, Boston: De Gruyter, 2021. https://doi.org/10.1515/9783110616880

2019

Bodle, Josephine; Hamouda, Mehdi; Cai, Shaobo; Williams, Ramey; Bernacki, Susan, and Loboa, Elizabeth. Primary Cilia Exhibit Mechanosensitivity to Cyclic Tensile Strain and Lineage-Dependent Expression in Adipose-Derived Stem Cells. Scientific Reports (2019) 9:8009
https://doi.org/10.1038/s41598-019-43351-y . Published online 29 May 2019.

Chen, Zhilong; Maimaiti, Rexiati; Zhu, Chaoqun; et al. Z-band and M-band titin splicing and regulation by RNA binding motif 20 in striated muscles. Journal of Cellular Biochemistry, Volume: 119, Issue: 12, Pages: 9986-9996. Published: Dec. 2018

Chen, Zhilong; Song, Jiangping; Chen, Liang; et al., Feb. 2018. Characterization of TTN Novex Splicing Variants across Species and the Role of RBM20 in Novex-Specific Exon Splicing. Genes. Volume: 9, Issue: 2. Article Number: 86.

Cruz-Teran, C. A., Bacon, K., and Rao, B. M. Simultaneous secretion and display of proteins in Saccharomyces cerevisiae (accepted in Methods in Molecular Biology 2019)

Cruz-Teran, C. A., Bacon, K., McArthur, N. and Rao, B. M., An engineered Sso7d variant enables efficient magnetization of yeast cells ACS Combinatorial Science 2018 20(10), 579-584

Farzaneh, M.; Attari, F.; Khoshnam, S. E.; et al., 2018. The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system. British Poultry Science, Volume: 59, Issue: 2. Pages: 240-244.

Fisher, S., Polyak, E., Zhang, X. Y., A bioreactor in virtual reality and video games to enhance the learning experience in bioprocess labs. Accepted by EDULEARN19 Proceedings, Barcelona, Spain, Jul 1-3, 2019

Islam, N., P.V. Gurgel, O.J. Rojas, and R.G. Carbonell, Use of a Branched Linker for Enhanced Biosensing Properties in IgG Detection from Mixed Chinese Hamster Ovary Cell Cultures, Bioconjugate Chemistry, 30(3), 815-825, 2019.

Islam, T., Naik, A.D., Hashimoto, Y., Menegatti, S., and R.G. Carbonell, Optimization of Sequence, Display and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality, International J. of Molecular Sciences, 20(1), 161, 2019.

Kish, W.S., Roach, M.K., Sachi, H., Naik, A.D., Menegatti, S., and R.G. Carbonell, Purification of Human Erythropoietin by Affinity Chromatography Using Cyclic Peptide Ligands, J. of Chromatography B, 1085, 1-12, 2018.

Maaenia, Y., M, S. B. Leeb, I.H. Ismaila, P. Mozdziak, and Y. S., Kim, 2018. Cloning of Japanese quail (Coturnix Japonica) follistatin and production of bioactive quail follistatin288 in E. coli. International Journal of Poultry Science 17: 22-28.

Naik, A.D., Islam, T., Terasaka, T., Ohara Y., Hashimoto, Y. Menegatti, S., and R.G. Carbonell, Silica Resins and Peptide Ligands to Develop Disposable Affinity Adsorbents for Antibody Purification, Biochemical Engineering Journal, Biochemical Engineering Journal, 145, 53-61, 2019.

Patil, R., Miles, J., Ko, Y., Datta, P., Rao, B. M., Kiserow, D., and Genzer J., Kinetic study of degrafting poly(methyl methacrylate) brushes from flat substrates by tetrabutylammonium fluoride Macromolecules 2018 51(24). 10237-10245

Pearce, K. H., Overton, L. K., Gampe, R. T., Barrett, G. B., Taylor, J. D., McKee, D. D., Campobasso, N., Nolte, R. T. & Reid, R. A. BacMam production and crystal structure of nonglycosylated apo human furin at 1.89 Å resolution. (2019). Acta Cryst. F75, 239-245. https://doi.org/10.1107/S2053230X19001419

Shoeibi, S., P. Mozdziak, and S. Mohammadi, 2018. Important signals regulating coronary artery angiogenesis. Journal of Microvascular Research 117: 1-9.

Stern RA, Mozdziak PE. 2019. Glutamine synthetase in avian muscle contributes to a positive myogenic response to ammonia compared to mammalian muscle.American Journal of Physiology. 10.1152/ajpregu.00232.2018

Stern, Rachel & Mozdziak, Paul. (2019). Differential ammonia metabolism and toxicity between avian and mammalian species, and effect of ammonia on skeletal muscle: A comparative review. Journal of Animal Physiology and Animal Nutrition. 10.1111/jpn.13080.

Tiruthani, K., Mischler A., Ahmed, S., Haugh, J. M.* and Rao, B. M.*, Design and evaluation of engineered protein biosensors for live cell imaging of Epidermal Growth Factor Receptor phosphorylation (accepted in Science Signaling 2019) *co-corresponding authors

Torkasvand, H., R. Fahti, A. Shahverdi, A. Golkar, P. E. Mozdziak, and H. Eimani, 2019. The effect of chick embryo extract on pre-antral follicles in vitro. Veterinary Research Forum (In Press).

Vann L, Sheppard JD. 2018. Cell-Culture-Based Influenza Vaccine Manufacturing: Evaluation of Near-Infrared Spectroscopy for In-Line Determination of Virus Titers. Bioprocess International (September) 16:34–43.

Zhang F, Xie Y, Celik H, Akkus O, Bernacki SH, King MW. 2019. Engineering small-caliber vascular grafts from collagen filaments and nanofibers with comparable mechanical properties to native vessels. Biofabrication 11. DOI: 10.1088/1758-5090/ab15ce

2018

Chen, Z., R. Maimaiti, C. Zhu, H. Cai, R. A. Stern, P. Mozdziak, Y. Ge, S. P. Ford, P.W. Nathanielsz, and W. Guo, 2018. Z-band and M-band titin splicing and regulation by RNA binding motif 20 in striated muscles. J Cell Biochem. https://doi.org/10.1002/jcb.27328

Chen, Z.; Song, J.; Chen, L.; Zhu, C.; Cai, H.; Sun, M.; Stern, A.; Mozdziak, P.; Ge, Y.; Means, W.J.; Guo, W. Characterization of TTN Novex Splicing Variants across Species and the Role of RBM20 in Novex-Specific Exon Splicing. Genes 2018, 9, 86.

Farzaneh, M., Attari, F., Khoshnam, S. E. & Mozdziak P. E., 2018. The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system, British Poultry Science, 59:2, 240-244, DOI: 10.1080/00071668.2017.1413234

Kalkreuter E, Carpenter SM, Williams GJ. 2018. Precursor-directed Biosynthesis and Semi-synthesis of Natural Products. In: Chemical and Biological Synthesis: Enabling Approaches for Understanding Biology. Royal Society of Chemistry. p. 275–312.

Kalkreuter E, Williams GJ. 2018. Engineering enzymatic assembly lines for the production of new antimicrobials.Current Opinion in Microbiology 45:140–148.

Kasey CM, Williams GJ. Customizing Transcription-factor Biosensors for Modern Biotechnology. RSC Catalysis Series No. 32, Modern Biocatalysis: Advances Towards Synthetic Biological Systems, 2018, 205.

Kasey CM, Zerrad M, Li Y, Cropp TA, Williams GJ. Development of transcription factor-based designer macrolide biosensors for metabolic engineering and synthetic biology. ACS Synth Biol, 2018, 7, 227.

Krieger, J. A. Yesinovskiy, P. Mozdziak, and M. Kim, 2018. Developing scaleable production methods for cultured pork and turkey meat: beyond. Proceedings of the Reciprocal Meat Conference.

Maaenia, Y., M, S. B. Leeb, I.H. Ismaila, P. Mozdziak, and Y. S., Kim, 2018. Cloning of Japanese quail (Coturnix Japonica) follistatin and production of bioactive quail follistatin288 in E. coli, International Journal of Poultry Science (In Press).

Madden AA, Epps MJ, Fukami T, Irwin RE, Sheppard J, Sorger DM, Dunn RR. 2018 The ecology of insect-yeast relationships and its relevance to human industry. Proc. R. Soc. B 285: 20172733. http://dx.doi.org/10.1098/rsbp.2017.2733

Nazari M, Malico AA, Ekelöf M, Lund S, Williams GJ, Muddiman DC. 2018. Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI.Analytical and Bioanalytical Chemistry 410:953–962.

Odum J, Flickinger M eds. 2018. Process Architecture in Biomanufacturing Facility Design. Hoboken, NJ: John Wiley and Sons.

Probst, S., Pancorbo, J., Pelletier, M., Lee Afanador, N., Ramalingam Iyer, K., Johnson, J.C. (2018) Data Driven Equipment and Facility Design Case Study: Funstioanl Closure of a Hybrid Connection System. BioPhorum Operations Group.

Shoeibia S, Mozdziak P, Mohammadi S. 2018. Important signals regulating coronary artery angiogenesis.Microvascular Research 117:1–9.

Torkasvand, H., R. Fahti, A. Shahverdi, A. Golkar, P.E. Mozdziak, and H. Eimani, 2018. The effect of chick embryo extract on pre-antral follicles in vitro. Veterinary Research Forum (In Press).

Zhang X.Y., 2018. Designing peer teaching using the backward design strategy and retrieval, spaced, and varied practices in undergraduate courses, 10th Annual Conference on Higher Education Pedagogy, Blacksburg, VA, Feb 14-16, 2018.

2017

Amid H, Maźe B, Flickinger MC, Pourdeyhimi B. 2017. Dynamic Adsorption of Ammonia: Apparatus, Testing Conditions and Adsorption Capacities. Measurement Science and Technology 28.

Bazgir B, Fathei R, Valoujerd MR, Mozdziak P, Asgari A. 2017. Satellite Cells Contribution to Exercise Mediated Muscle Hypertrophy and Repair: A Review Article. Cell Journal 18:473–484.

Bernal OI, Bharti B, Flickinger MC, Velev OD. 2017. Fabrication of Photoreactive Biocomposite Surfaces via Electric Field Assisted Assembly of Cyanobacteria. Langmuir 33:5304–5313.

Bernal OI, Pawlak JJ, Flickinger MC. 2017. Microbial Paper: A Cellulose Fiber Self-Tuning Photoabsorber Producing Hydrogen Gas from Acetate Using Dry-Stabilized Rhodopseudomonas palustris. BioResource 12:4013–4030.

Cai S, Bodle JC, Mathieu PS, Amos A, Hamouda M, Bernacki S, McCarty G, Loboa EG. 2017. Primary Cilia are Sensors of Electrical Field Stimulation to Induce Osteogenesis of Human Adipose Derived Stem Cells. FASEB Journal 31:346–355.

Cortez SM, Nicolau APMRC, Flickinger MC, Mota MJMG. 2017. Biocoatings: A New Challenge for Environmental Biotechnology. Biochemical Engineering Journal 121:25–37.

Farzaneh M, Hossani SN, Mozdziak PE, Baharvand H. 2017. Avian Embryos and Related Cell Lines: A Convenient Platform for Recombinant Proteins and Vaccines Production. Biotechnology Journal 12.

Farzaneh M, Khoshnam SE, Mozdziak PE. 2017. Concise Review: Avian Multipotent Stem Cells as a Novel Tool for Investigating Cell Based Therapies. Journal of Dairy, Animal and Veterinary Research 5:804–810. DOI: 10.15406/jdvar.2017.05.00125.

Flickinger MC, Bernal OI, Schulte MJ, Broglie JJ, Duran CJ, Wallace A, Mooney CB, Velev OD. 2017. Biocoatings: Challenges to Expanding the Functionality of Waterborne Latex Coatings by Incorporating Concentrated Living Microorganisms. Journal of Coatings Technology and Research 14:791–808.

Kish, WS, H. Sachi, A.D. Naik, et al., Design, Selection, and Development of Cyclic Peptide Ligands for Human Erythropoeitin, 2017, J. of Chromatography A, 1500, 105-120.

Lawless, R. Crossing Over to Large Molecules Bridges to Biomanufacturing, 2017, Pharmaceutical Engineering.

Lawless, Rick. Building Competency in Basic Science: The Secret Weapon of Tomorrow's Bioprocessing Technician, 2017. BioProcess International.

Liu, X., W. Yuan, W, Sharma-Shivappa, R., and van Zanten, J.H. 2017. Antioxidant Activity of Phlorotannins from Brown Algae. , Int J Agric & Biol Eng 10 184-191.

Musiol-Kroll EM, Zubeil F, Schafhauser T, Härtner T, Kulik A, McArthur JB, Koryakina I, Wohlleben W, Grond S, Williams GJ, Lee SY, and Weber T. “Polyketide bio-derivatization using the promiscuous acyltransferase KirCII”. ACS Synth Biol, 2017, 6, 421.

Nappo M, Hamilton PT. 2017. Developing Career Self Efficacy through Biotech Industry Practicum Projects. The Innovator 10:19–23.

Schulte, M. J., Solocinski, J.,Wang, M., Kovacs, M., Kilgore, R., Flickinger, M. C., and Chakraborty, N. “A Method for Quantifying Residual Moisture in Paper Biocomposite Biocatalysts using Raman Microspectroscopy” PLoS One (2017) doi.org/10.1371/journal.pone.0180806.

Shoeibi, S., P. Mozdziak, A. Golkar-Narenji, 2017. Biogenesis of selenium nanoparticles using green chemistry. Topics in Current Chemistry 375: 88

Stern RA, Dasarathy S, Mozdziak PE. 2017. Ammonia Elicits a Different Myogenic Response in Avian and Murine Myotubes. In Vitro Cellular and Developmental Biology 53:99–110.

Vann L, Layfield JB, Sheppard JD. 2017. The Application of Near-Infrared Spectroscopy (NIRS) in Beer Fermentation for On-line Monitoring of Critical Process Parameters and their Integration into a Novel Feedforward Control Strategy. Journal of the Institute of Brewing 123.

Whitham JM, Schulte MJ, Bobay BG, Bruno-Barcena JM, Chinn MS, Flickinger MC, Pawlak JC, Grunden AM. 2017. Characterization of Clostridium ljungdahlii OTA1: A Non-Autotrophic Hyper Ethanol-Producing Strain. Applied Microbiology and Biotechnology 101:1615–1630.

Zhang X.Y., Polyak E. 2017. Stories for Science Classes: Experimental Interactive Metaphors. EDULEARN17 Proceedings. Barcelona.2017, 1332-1339.

2016

Amid H, Maze B, Flickinger MC, Pourdeyhimi B. 2016. Hybrid Adsorptive Nonwoven Structures: A Review of Current Technologies. Journal of Materials Science 51:4173–4200.

Gilleskie GL. 2016. Process-Oriented Training in Single-Use Technology. BioProcess International Supplement Series 14:14–16.

Gilleskie GL, Reeves B, Van Zanten JH, Carbonell R, Balchunas J. 2016. A “Life Cycle” Approach to Education and Training for the Biopharmaceutical Industry. Chemical Engineering Education 50:193–201.

Heller M, Wimbish R, Gurgel PV, Pourdeyhimi B, Carbonell RG. 2016. Reducing Diffusion Limitations in Ion Exchange Grafted Membranes Using High Surface Area Nonwovens. Journal of Membrane Science 514:53–64.

Menegatti S, Bobay B, Ward K, Islam T, Kish W, Naik AD, Carbonell RG. 2016. Design of Protease-Resistant Peptide Ligands for Purification of Antibodies from Human Plasma. Journal of Chromatography A 1445:93–104.

Mosley BJ, Hentz N. 2016. Development of a Stability Indicating Method for Green Fluorescent Protein by HPLC. LCGC North America 34:798–803.

Mozdziak P, Gibbons M. Mass Production of Avian Muscle Cells. Proceedings 2nd International Conference on Cultured Meat Post M, editor. 2016.

Oezcam AE, Efimenko K, Spontak RJ. 2016. Multipurpose Polymeric Coating for Functionalizing Inert Polymer Surfaces. ACS Applied Materials and Interfaces 8:5694–5705.

Schulte MJ, Wiltgen J, RItter J, Mooney CB, Flickinger MC. 2016. A High Gas Fraction Reduced Power Bioprocessing Method Demonstrated with a Clostridium ljungdahlii OTA1 Paper Biocomposite. Biotechnology and Bioengineering 113:1913–1923.

Shen F, Rojas OJ, Genzer J, Gurgel PV, Carbonell RG. 2016. Affinity Interactions of Human Immunoglobulin G with Short Peptides: Role of Ligand Spacer on Binding, Kinetics, and Mass Transfer. Analytical and Bioanalytical Chemistry 408:1829–1841.

Van Bergen B, Cyr N, Strasser R, Blanchette M, Sheppard JD, Jardim A. 2016. Alpha, Beta Dicarbonyl Reduction is Mediated by the Saccharomyces Old Yellow Enzyme. FEMS Yeast Research 16:1–12.

Zandvakili M, Eimani H, Golkar-Narenji A, Eftekhari P, Shahverdi A, Mozdziak PE. 2016. Histological Evaluations of the Effect of VEGF on Auto-Transplanted Mouse Ovaries. Animal Cells and Systems 20:260–266.

2015

Bozdag A, Komives C, Flickinger MC. 2015. Growth of Bacillus methanolicus on 2 M Methanol at 50°C: The Effect of High Methanol Concentration on Gene Regulation of Enzymes Involved in Formaldehyde Detoxification by the Ribulose Monophosphate Pathway. Journal of Industrial Microbiology and Biotechnology 42:1027–1038.

Estrada JM, Bernal OI, Flickinger MC, Muñoz R, Deshusses M. 2015. Biocatalytic Coatings for Air Pollution Control: Proof of Concept Study on Enhancing the Rate of VOC Biodegradation. Biotechnology and Bioengineering 112:263–271.

Gilleskie GL, Brown P. 2015. Comprehensive Hands-on Training for Biopharmaceutical Manufacturing: BTEC’s Program to Deliver Training to FDA Investigators. BioProcess International 13:12–21.

Johnson MW, Golden K, Gilleskie G, Pancorbo J, Vilhena RR. 2015. Comparison of Concentration Measurement Technologies in Bioprocess Solutions. BioProcess International 13:60–67.

Karoshi G, Kolar P, Shah SB, Gilleskie G, Das L. 2015. Calcined Eggshell as an Inexpensive Catalyst for Partial Oxidation of Methane. Journal of the Taiwan Institute of Chemical Engineers 57:123–128.

Layfield JB, Sheppard JD. 2015. What Brewers Should Know About Viability, Vitality, and Overall Brewing Fitness: A Mini-Review. MBAA Technical Quarterly 52:132–140.

Liu HY, Gurgel PV, Carbonell RG. 2015. Preparation and Characterization of Anion Exchange Adsorptive Membranes with High Protein Binding Capacity. Journal of Membrane Science 493:349–359.

Stern RA, Ashwell CM, Dasarathy S, Mozdziak PE. 2015. The Effect of Hyperammonemia on Myostatin and Myogenic Regulatory Factor Gene Expression in Broiler Embryos. Animal 9:992–999.

Tambe N, Di J, Zhang Z, Bernacki S, El-Shafei A, King M. 2015. Novel Genipin-Collagen Immobilization of Polylactic Acid (PLA) Fibers for Use as Tissue Engineering Scaffolds. Journal of Biomedical Materials Research Part B: Applied Biomaterials 103:1188–1197.

2014

Azizoglu RO, Elhanafi D, Kathariou S. 2014. Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes. Methods in Molecular Biology 1157:201–211.

Bernal OI, Mooney CB, Flickinger MC. 2014. Specific Photosynthetic Rate Enhancement by Cyanobacteria Coated onto Paper Enables Engineering of Highly Reactive Cellular Biocomposite “Leaves.” Biotechnology and Bioengineering 111:1993–2008.

Billakanti JM, Fee C, Naik AD, Carbonell RG. 2014. Application of Peptide Chromatography for the Isolation of Antibodies from Bovine Skim Milk, Acid Whey and Colostrum. Food and Bioproducts Processing 92:199–207.

Bodle J, Teeter S, Hluck B, Hardin J, Bernacki S, Loboa E. 2014. Age-Related Effects on the Potency of Human Adipose Derived Stem Cells: Creation and Evaluation of Superlots and Implications for Musculoskeletal Tissue Engineering Applications. Tissue Engineering Part C Methods 20:972–983.

Dutta V, Elhanafi D, Kathariou S. 2013. Conservation and Distribution of the Benzalkonium chloride Resistance Cassette bcrABC in Listeria monocytogenes. Applied Environmental Microbiology 79:6067–6074.

Dutta V, Elhanafi D, Osborne J, Martinez MR, Kathariou S. 2014. Genetic Characterization of Plasmid-Associated Triphenylmethane Reductase in Listeria monocytogenes. Applied and Environmental Microbiology 80:5379–5385.

Fidaleo M, Bortone N, Schulte N, Flickinger MC. 2014. Ink-Jet Printing of Gluconobacter oxydans Micropatterned Coatings as High Surface-to-Volume Ratio Bio-Reactive Coatings. Coatings 4:1–17.

Gilleskie GL, Reeves B. 2014. From Process Development to Manufacturing: Lab-intensive Courses in Downstream Bioprocessing. Chemical Engineering Education 48:79.

Hentz N, Knaide T. 2014. Effect of Liquid-Handling Accuracy on Assay Performance. Journal of Laboratory Automation 19:153–162.

Islam N, Shen F, Gurgel PV, Rojas OJ, Carbonell RG. 2014. Dynamic and Equilibrium Performance of Sensors Based on Short Peptide Ligands for Affinity Adsorption of IgG Using Surface Plasmon Resonance. Biosensors and Bioelectronics 58:380–387.

Islam N, Gurgel PV, Rojas OJ, Carbonell RG. 2014. Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobin Detection. Journal of Physical Chemistry V 118:5361–5373.

Layfield JB, Vann LR, Sheppard JD. 2014. A Novel method of Inducing and Retaining Cell Cycle Synchronization in Cultures of Saccharomyces cerevisiae. Journal of the American Society of Brewing Chemists 72:102–109.

Karoshi G, Kolar P, Shah SB, Gilleskie G. 2014. Value-Addition of Methane Via Selective Catalytic Oxidation. Biofuels 5:175–188.

Ruiz J, Gilleskie GL, Brown P, Burnett B, Carbonell RG. 2014. Comprehensive Hands-on Training for Influenza Vaccine Manufacturing: A WHO-BARDA-BTEC Partnerships for Workforce Development. Biochemistry and Biology Education 42:414–419.

2013

Broglie JJ, Flickinger MC, Velev OD. 2013. Continuous-Sedimentation Assembly of Colloidal Microsphere Coatings for Biotechnology Applications. Coatings 3:26–48.

Clontz L, Gilleskie G, Hentz N. 2013. Biopharmaceutical Microbial Contamination Control: Processes, Equipment, Facilities, and Systems to Prevent Microbial Contamination. Pharmaceutical Processing 28:16–21.

Efimenko K, Özçam AE, Fischer DA, Phelan FR, Douglas JF. 2013. Self-Assembly Fronts in Collision: Impinging Ordering Organosilane Layers. Soft Matter 8:2493–2505.

Flickinger M ed. 2013. In: Downstream Industrial Biotechnology: Recovery and Purification. Hoboken, NJ: John Wiley & Sons.

Flickinger M ed. 2013. Upstream Industrial Biotechnology: Expression Systems and Process Development (volume 1); Equipment, Process Design, Sensing and Control and cGMP Operations (volume 2). Hoboken, NJ: John Wiley & Sons.

Ford JP, Thapaliya N, Kelly MJ, Roberts WL, Lamb HH. 2013. Semi-Batch Deoxygenation of Canola- and Lard-Derived Fatty Acids to Diesel-Range Hydrocarbons. Energy Fuels 27:7489–7496.

Gray SR, Peretti SW, Lamb HH. 2013. Real-Time Monitoring of High-Gravity Corn Mash Fermentation Using In Situ Raman Spectroscopy. Biotechnology and Bioengineering 110:1654–1662.

Jenkins JS, Flickinger MC, Velev OD. 2013. Engineering Cellular Composite Materials Using Convective Assembly. Materials 6:1803–1825.

Kish WS, Naik AD, Menegatti S, Carbonell RG. 2013. Peptide-Based Affinity Adsorbents with High Binding Capacity for the Purification of Monoclonal Antibodies. Industrial and Engineering Chemistry Research 52:8800–8811.s

Liu Z, Gurgel PV, Carbonell RG. 2013. Affinity Chromatographic Purification of Human Immunoglobulin A from Chinese Hamster Ovary Cell Culture Supernatant. Biotechnology Progress 29:91–98.

Liu Z, Gurgel PV, Carbonell RG. 2013. Affinity Chromatographic Purification of Human Immunoglobulin M from Human B Lymphocyte Cell Culture Supernatant. Biotechnology Progress 70:53–70.

Liu H, Zheng Y, Gurgel PV, Carbonell RG. 2013. Affinity Membrane Development from PBT Nonwoven by Photo-Induced Graft Polymerization, Hydrophilization and Ligand Attachment. Journal of Membrane Science 428:562–575.

Menegatti S, Hussain M, Naik AD, Carbonell RG, Rao BM. 2013. mRNA Display Selection and Solid-Phase Synthesis of Fc-Binding Cyclic Peptide Affinity Ligands. Biotechnology and Bioengineering 110:857–870.

Menegatti S, Naik AD, Carbonell RG. 2013. The Hidden Potential of Small Synthetic Molecules and peptides as Affinity Ligands for Bioseparations. Pharmaceutical Bioprocessing 1:467–485.

Menegatti S, Ward KL, Naik AD, Kish WS, Blackburn RK, Carbonell RG. 2013. Reversible Cyclic Peptide Libraries for the Discovery of Affinity Ligands. Analytical Chemistry 85:9229–9237.

Mocka E, Stern R, Fletcher O, Anderson K, Petitte JN, Mozdiziak PE. 2013. Rescue of Mutant p53 by CP-31398 Prevents Spontaneous Ovarian Cancer to the Domestic Hen. Graduate Student Research Symposium at NC State :6.

Peng Q, Efimenko K, Genzer J, Parsons GN. 2012. Oligomer Orientation in Vapor-Molecular-Layer-Deposited Alkyl-Aromatic Polyamide Films. Langmuir 28:10464–10470.

Pikorska M, Soule T, Gosse JL, Milliken C, Smith GW, Yeager CM. 2013. Preservation of H2 Production Activity in Nanoporous Latex Coatings of Rhodopseudomonas palustris CGA009 during Dry Storage at Ambient Temperatures. Microbial Biotechnology 6:515–525.

Stallings KD, Kitchener RL, Hentz NG. 2014. A High-Temperature, High Throughput Method for Monitoring Residual Formaldehyde in Vaccine Formulations. Journal of Laboratory Automation 19:275–284.

Susanti RF, Han YS, Kim J, Lee YH, Carbonell RG. 2013. A New Strategy for Ultralow Biofouling Membranes: Uniform and Ultrathin Hydrophilic Coatings Using Liquid Carbon Dioxide. Journal of Membrane Science 440:88–97.

Wang E, Allen A, Campos A, Killens Cade R, Dean L, Dvora M, Immer JG, Mixson S, Srirangan S, Sauer S, et al. 2013. ASI: Dunaliella Marine Microalgae to Drop-In Replacement Liquid Transportation Fuel. Environmental Progress and Sustainable Energy 32:916–925.

Wilson AR, Sun K, Chi M, White R, LeBeau JM, Lamb HH, Wiley BJ. 2013. From Core-Shell to Alloys: The Preparation and Characterization of Solution-Synthesized AuPd Nanoparticle Catalysts. Journal of Physical Chemistry C 117:7489–7496.

Witcher M, Carbonell RG, Odum J, Bigelow P, Lewis P, Zivitz M. 2013. Facility of the Future: Next Generation Biomanufacturing Forum, Part I. Pharmaceutical Engineering 30:1–7.

Witcher M, Carbonell RG, Odum J, Bigelow P, Lewis P, Zivitz M. 2013. Facility of the Future: Next Generation Biomanufacturing Forum, Part II. Pharmaceutical Engineering 30:1–7.

Zhang Y, Carbonell RG, Rojas OJ. 2013. Bioactive Cellulose Nanofibrils for Specific Human IgG Binding. Biomacromolecules 14:4161–4168.

Zhang Y, Islam N, Carbonell RG, Rojas OJ. 2013. Specific Binding of Immunoglobulin G with Bioactive Short Peptides Supported on Antifouling Copolymer Layers for Detection in Quartz Crystal Microgravimetry and Surface Plasmon Resonance. Analytical Chemistry 85:1106–1113.

Zhang Y, Islam N, Carbonell RG, Rojas OJ. 2013. Specificity and Regenerability of Short Peptide Ligands Supported on Polymer Layers for Immunoglobulin G Binding and Detection. ACS Applied Materials and Interfaces 5:8030–8037.

2012

Bonino CA, Efimenko K, Jeong SI, Krebs MD, Alsberg E, Khan SA. 2012. Three-Dimensional Electrospun Alginate Nanofiber Mats via Tailored Charge Repulsions. Small 8:1928–1936.

Datta P, Efimenko K, Genzer J. 2012. The Effect of Confinement on Thermal Frontal Polymerization. Polymer Chemistry 12:3243–3246.

Flickinger M ed. 2012. Advancing Coatings with Biotechnology. In: Coatings.

Gera N, Hill AB, White DP, Carbonell RG, Rao BM. 2012. Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold. PLoS ONE 7.

Gosse JL, Chinn MS, Grunden AM, Flickinger MC. 2012. A Versatile Method for the Preparation of Hydrated Microbial-latex Biocatalytic Coatings for Headspace Gas Absorption and Gas Evolution. Journal of Industrial Microbiology and Biotechnology 39:1269–1278.

Heldt CL, Gurgel PV, Jaykus L-A, Carbonell RG. 2012. Porcine Parvovirus Removal Using Trimer and Biased Hexamer Peptides. Biotechnology Journal 7:558–565.

Jenkins JS, Flickinger MC, Velev OD. 2012. Deposition of Composite Coatings from Particle-Particle and Particle-Yeast Cell Blends by Convective-Sedimentation Assembly. Journal of Colloid and Interface Science 380:192–200.

Katharios-Lanwermeyer S, Rakic-Martinez M, Elhanafi D, Ratani S, Tiedje JM, Kathariou S. 2012. Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae. Applied and Environmental Microbiology 78:7549–7556.

Menegatti S, Naik AD, Gurgel PV, Carbonell RG. 2012. Alkaline-Stable Peptide Ligand Affinity Adsorbents for the Purification of Biomolecules. Journal of Chromatography A 1245:55–64.

Menegatti S, Naik AD, Gurgel PV, Carbonell RG. 2012. Purification of Polyclonal Antibodies from Cohn Fraction II + III, Skim Milk, and Whey by Affinity Chromatography Using a Hexamer Peptide Ligand. Journal of Separation Science 35:3139–3148.

Mota M, Flickinger MC. 2012. Modeling the Influence of Slurry Concentration on Saccharomyces cerevisiae Cake Porosity and Resistance During Microfiltration. Biotechnology Progress 28:1534–1541.

Naik AD, Menegatti S, Reese HR, Gurgel PV, Carbonell RG. 2012. Process for Purification of Monoclonal Antibody Expressed in Transgenic Lemna Plant Extract Using Dextran-Coated Charcoal and a Hexamer Peptide Affinity Resin. Journal of Chromatography A 1260:61–66.