Recent publications by BTEC faculty and staff

Each year, faculty and instructional staff affiliated with BTEC publish numerous articles in both peer-reviewed journals and industry publications. Publications for the past several years are listed below in reverse chronological order.


Amid H, Maźe B, Flickinger MC, Pourdeyhimi B. 2017. Dynamic Adsorption of Ammonia: Apparatus, Testing Conditions and Adsorption Capacities. Measurement Science and Technology 28.

Bazgir B, Fathei R, Valoujerd MR, Mozdziak P, Asgari A. 2017. Satellite Cells Contribution to Exercise Mediated Muscle Hypertrophy and Repair: A Review Article. Cell Journal 18:473–484.

Bernal OI, Bharti B, Flickinger MC, Velev OD. 2017. Fabrication of Photoreactive Biocomposite Surfaces via Electric Field Assisted Assembly of Cyanobacteria. Langmuir 33:5304–5313.

Bernal OI, Pawlak JJ, Flickinger MC. 2017. Microbial Paper: A Cellulose Fiber Self-Tuning Photoabsorber Producing Hydrogen Gas from Acetate Using Dry-Stabilized Rhodopseudomonas palustris. BioResource 12:4013–4030.

Cai S, Bodle JC, Mathieu PS, Amos A, Hamouda M, Bernacki S, McCarty G, Loboa EG. 2017. Primary Cilia are Sensors of Electrical Field Stimulation to Induce Osteogenesis of Human Adipose Derived Stem Cells. FASEB Journal 31:346–355.

Cortez SM, Nicolau APMRC, Flickinger MC, Mota MJMG. 2017. Biocoatings: A New Challenge for Environmental Biotechnology. Biochemical Engineering Journal 121:25–37.

Farzaneh M, Hossani SN, Mozdziak PE, Baharvand H. 2017. Avian Embryos and Related Cell Lines: A Convenient Platform for Recombinant Proteins and Vaccines Production. Biotechnology Journal 12.

Farzaneh M, Khoshnam SE, Mozdziak PE. 2017. Concise Review: Avian Multipotent Stem Cells as a Novel Tool for Investigating Cell Based Therapies. Journal of Dairy, Animal and Veterinary Research 5:804–810. DOI: 10.15406/jdvar.2017.05.00125.

Flickinger MC, Bernal OI, Schulte MJ, Broglie JJ, Duran CJ, Wallace A, Mooney CB, Velev OD. 2017. Biocoatings: Challenges to Expanding the Functionality of Waterborne Latex Coatings by Incorporating Concentrated Living Microorganisms. Journal of Coatings Technology and Research 14:791–808.

Stern RA, Dasarathy S, Mozdziak PE. 2017. Ammonia Elicits a Different Myogenic Response in Avian and Murine Myotubes. In Vitro Cellular and Developmental Biology 53:99–110.

Vann L, Layfield JB, Sheppard JD. 2017. The Application of Near-Infrared Spectroscopy (NIRS) in Beer Fermentation for On-line Monitoring of Critical Process Parameters and their Integration into a Novel Feedforward Control Strategy. Journal of the Institute of Brewing 123.

Whitham JM, Schulte MJ, Bobay BG, Bruno-Barcena JM, Chinn MS, Flickinger MC, Pawlak JC, Grunden AM. 2017. Characterization of Clostridium ljungdahlii OTA1: A Non-Autotrophic Hyper Ethanol-Producing Strain. Applied Microbiology and Biotechnology 101:1615–1630.

Zhang X.Y., Polyak E. 2017. Stories for Science Classes: Experimental Interactive Metaphors. EDULEARN17 Proceedings. Barcelona. 2017, 1332-1339.


Amid H, Maze B, Flickinger MC, Pourdeyhimi B. 2016. Hybrid Adsorptive Nonwoven Structures: A Review of Current Technologies. Journal of Materials Science 51:4173–4200.

Gilleskie GL. 2016. Process-Oriented Training in Single-Use Technology. BioProcess International Supplement Series 14:14–16.

Gilleskie GL, Reeves B, Van Zanten JH, Carbonell R, Balchunas J. 2016. A “Life Cycle” Approach to Education and Training for the Biopharmaceutical Industry. Chemical Engineering Education 50:193–201.

Heller M, Wimbish R, Gurgel PV, Pourdeyhimi B, Carbonell RG. 2016. Reducing Diffusion Limitations in Ion Exchange Grafted Membranes Using High Surface Area Nonwovens. Journal of Membrane Science 514:53–64.

Menegatti S, Bobay B, Ward K, Islam T, Kish W, Naik AD, Carbonell RG. 2016. Design of Protease-Resistant Peptide Ligands for Purification of Antibodies from Human Plasma. Journal of Chromatography A 1445:93–104.

Mosley BJ, Hentz N. 2016. Development of a Stability Indicating Method for Green Fluorescent Protein by HPLC. LCGC North America 34:798–803.

Mozdziak P, Gibbons M. Mass Production of Avian Muscle Cells. Proceedings 2nd International Conference on Cultured Meat Post M, editor. 2016.

Oezcam AE, Efimenko K, Spontak RJ. 2016. Multipurpose Polymeric Coating for Functionalizing Inert Polymer Surfaces. ACS Applied Materials and Interfaces 8:5694–5705.

Schulte MJ, Wiltgen J, RItter J, Mooney CB, Flickinger MC. 2016. A High Gas Fraction Reduced Power Bioprocessing Method Demonstrated with a Clostridium ljungdahlii OTA1 Paper Biocomposite. Biotechnology and Bioengineering 113:1913–1923.

Shen F, Rojas OJ, Genzer J, Gurgel PV, Carbonell RG. 2016. Affinity Interactions of Human Immunoglobulin G with Short Peptides: Role of Ligand Spacer on Binding, Kinetics, and Mass Transfer. Analytical and Bioanalytical Chemistry 408:1829–1841.

Van Bergen B, Cyr N, Strasser R, Blanchette M, Sheppard JD, Jardim A. 2016. Alpha, Beta Dicarbonyl Reduction is Mediated by the Saccharomyces Old Yellow Enzyme. FEMS Yeast Research 16:1–12.

Zandvakili M, Eimani H, Golkar-Narenji A, Eftekhari P, Shahverdi A, Mozdziak PE. 2016. Histological Evaluations of the Effect of VEGF on Auto-Transplanted Mouse Ovaries. Animal Cells and Systems 20:260–266.


Bozdag A, Komives C, Flickinger MC. 2015. Growth of Bacillus methanolicus on 2 M Methanol at 50°C: The Effect of High Methanol Concentration on Gene Regulation of Enzymes Involved in Formaldehyde Detoxification by the Ribulose Monophosphate Pathway. Journal of Industrial Microbiology and Biotechnology 42:1027–1038.

Estrada JM, Bernal OI, Flickinger MC, Muñoz R, Deshusses M. 2015. Biocatalytic Coatings for Air Pollution Control: Proof of Concept Study on Enhancing the Rate of VOC Biodegradation. Biotechnology and Bioengineering 112:263–271.

Gilleskie GL, Brown P. 2015. Comprehensive Hands-on Training for Biopharmaceutical Manufacturing: BTEC’s Program to Deliver Training to FDA Investigators. BioProcess International 13:12–21.

Johnson MW, Golden K, Gilleskie G, Pancorbo J, Vilhena RR. 2015. Comparison of Concentration Measurement Technologies in Bioprocess Solutions. BioProcess International 13:60–67.

Karoshi G, Kolar P, Shah SB, Gilleskie G, Das L. 2015. Calcined Eggshell as an Inexpensive Catalyst for Partial Oxidation of Methane. Journal of the Taiwan Institute of Chemical Engineers 57:123–128.

Layfield JB, Sheppard JD. 2015. What Brewers Should Know About Viability, Vitality, and Overall Brewing Fitness: A Mini-Review. MBAA Technical Quarterly 52:132–140.

Liu HY, Gurgel PV, Carbonell RG. 2015. Preparation and Characterization of Anion Exchange Adsorptive Membranes with High Protein Binding Capacity. Journal of Membrane Science 493:349–359.

Stern RA, Ashwell CM, Dasarathy S, Mozdziak PE. 2015. The Effect of Hyperammonemia on Myostatin and Myogenic Regulatory Factor Gene Expression in Broiler Embryos. Animal 9:992–999.

Tambe N, Di J, Zhang Z, Bernacki S, El-Shafei A, King M. 2015. Novel Genipin-Collagen Immobilization of Polylactic Acid (PLA) Fibers for Use as Tissue Engineering Scaffolds. Journal of Biomedical Materials Research Part B: Applied Biomaterials 103:1188–1197.


Azizoglu RO, Elhanafi D, Kathariou S. 2014. Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes. Methods in Molecular Biology 1157:201–211.

Bernal OI, Mooney CB, Flickinger MC. 2014. Specific Photosynthetic Rate Enhancement by Cyanobacteria Coated onto Paper Enables Engineering of Highly Reactive Cellular Biocomposite “Leaves.” Biotechnology and Bioengineering 111:1993–2008.

Billakanti JM, Fee C, Naik AD, Carbonell RG. 2014. Application of Peptide Chromatography for the Isolation of Antibodies from Bovine Skim Milk, Acid Whey and Colostrum. Food and Bioproducts Processing 92:199–207.

Bodle J, Teeter S, Hluck B, Hardin J, Bernacki S, Loboa E. 2014. Age-Related Effects on the Potency of Human Adipose Derived Stem Cells: Creation and Evaluation of Superlots and Implications for Musculoskeletal Tissue Engineering Applications. Tissue Engineering Part C Methods 20:972–983.

Dutta V, Elhanafi D, Kathariou S. 2013. Conservation and Distribution of the Benzalkonium chloride Resistance Cassette bcrABC in Listeria monocytogenes. Applied Environmental Microbiology 79:6067–6074.

Dutta V, Elhanafi D, Osborne J, Martinez MR, Kathariou S. 2014. Genetic Characterization of Plasmid-Associated Triphenylmethane Reductase in Listeria monocytogenes. Applied and Environmental Microbiology 80:5379–5385.

Fidaleo M, Bortone N, Schulte N, Flickinger MC. 2014. Ink-Jet Printing of Gluconobacter oxydans Micropatterned Coatings as High Surface-to-Volume Ratio Bio-Reactive Coatings. Coatings 4:1–17.

Gilleskie GL, Reeves B. 2014. From Process Development to Manufacturing: Lab-intensive Courses in Downstream Bioprocessing. Chemical Engineering Education 48:79.

Hentz N, Knaide T. 2014. Effect of Liquid-Handling Accuracy on Assay Performance. Journal of Laboratory Automation 19:153–162.

Islam N, Shen F, Gurgel PV, Rojas OJ, Carbonell RG. 2014. Dynamic and Equilibrium Performance of Sensors Based on Short Peptide Ligands for Affinity Adsorption of IgG Using Surface Plasmon Resonance. Biosensors and Bioelectronics 58:380–387.

Islam N, Gurgel PV, Rojas OJ, Carbonell RG. 2014. Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobin Detection. Journal of Physical Chemistry V 118:5361–5373.

Layfield JB, Vann LR, Sheppard JD. 2014. A Novel method of Inducing and Retaining Cell Cycle Synchronization in Cultures of Saccharomyces cerevisiae. Journal of the American Society of Brewing Chemists 72:102–109.

Karoshi G, Kolar P, Shah SB, Gilleskie G. 2014. Value-Addition of Methane Via Selective Catalytic Oxidation. Biofuels 5:175–188.

Ruiz J, Gilleskie GL, Brown P, Burnett B, Carbonell RG. 2014. Comprehensive Hands-on Training for Influenza Vaccine Manufacturing: A WHO-BARDA-BTEC Partnerships for Workforce Development. Biochemistry and Biology Education 42:414–419.


Broglie JJ, Flickinger MC, Velev OD. 2013. Continuous-Sedimentation Assembly of Colloidal Microsphere Coatings for Biotechnology Applications. Coatings 3:26–48.

Clontz L, Gilleskie G, Hentz N. 2013. Biopharmaceutical Microbial Contamination Control: Processes, Equipment, Facilities, and Systems to Prevent Microbial Contamination. Pharmaceutical Processing 28:16–21.

Efimenko K, Özçam AE, Fischer DA, Phelan FR, Douglas JF. 2013. Self-Assembly Fronts in Collision: Impinging Ordering Organosilane Layers. Soft Matter 8:2493–2505.

Flickinger M ed. 2013. In: Downstream Industrial Biotechnology: Recovery and Purification. Hoboken, NJ: John Wiley & Sons.

Flickinger M ed. 2013. Upstream Industrial Biotechnology:  Expression Systems and Process Development (volume 1); Equipment, Process Design, Sensing and Control and cGMP Operations (volume 2). Hoboken, NJ: John Wiley & Sons.

Ford JP, Thapaliya N, Kelly MJ, Roberts WL, Lamb HH. 2013. Semi-Batch Deoxygenation of Canola- and Lard-Derived Fatty Acids to Diesel-Range Hydrocarbons. Energy Fuels 27:7489–7496.

Gray SR, Peretti SW, Lamb HH. 2013. Real-Time Monitoring of High-Gravity Corn Mash Fermentation Using In Situ Raman Spectroscopy. Biotechnology and Bioengineering 110:1654–1662.

Jenkins JS, Flickinger MC, Velev OD. 2013. Engineering Cellular Composite Materials Using Convective Assembly. Materials 6:1803–1825.

Kish WS, Naik AD, Menegatti S, Carbonell RG. 2013. Peptide-Based Affinity Adsorbents with High Binding Capacity for the Purification of Monoclonal Antibodies. Industrial and Engineering Chemistry Research 52:8800–8811.s

Liu Z, Gurgel PV, Carbonell RG. 2013. Affinity Chromatographic Purification of Human Immunoglobulin A from Chinese Hamster Ovary Cell Culture Supernatant. Biotechnology Progress 29:91–98.

Liu Z, Gurgel PV, Carbonell RG. 2013. Affinity Chromatographic Purification of Human Immunoglobulin M from Human B Lymphocyte Cell Culture Supernatant. Biotechnology Progress 70:53–70.

Liu H, Zheng Y, Gurgel PV, Carbonell RG. 2013. Affinity Membrane Development from PBT Nonwoven by Photo-Induced Graft Polymerization, Hydrophilization and Ligand Attachment. Journal of Membrane Science 428:562–575.

Menegatti S, Hussain M, Naik AD, Carbonell RG, Rao BM. 2013. mRNA Display Selection and Solid-Phase Synthesis of Fc-Binding Cyclic Peptide Affinity Ligands. Biotechnology and Bioengineering 110:857–870.

Menegatti S, Naik AD, Carbonell RG. 2013. The Hidden Potential of Small Synthetic Molecules and peptides as Affinity Ligands for Bioseparations. Pharmaceutical Bioprocessing 1:467–485.

Menegatti S, Ward KL, Naik AD, Kish WS, Blackburn RK, Carbonell RG. 2013. Reversible Cyclic Peptide Libraries for the Discovery of Affinity Ligands. Analytical Chemistry 85:9229–9237.

Mocka E, Stern R, Fletcher O, Anderson K, Petitte JN, Mozdiziak PE. 2013. Rescue of Mutant p53 by CP-31398 Prevents Spontaneous Ovarian Cancer to the Domestic Hen. Graduate Student Research Symposium at NC State :6.

Peng Q, Efimenko K, Genzer J, Parsons GN. 2012. Oligomer Orientation in Vapor-Molecular-Layer-Deposited Alkyl-Aromatic Polyamide Films. Langmuir 28:10464–10470.

Pikorska M, Soule T, Gosse JL, Milliken C, Smith GW, Yeager CM. 2013. Preservation of H2 Production Activity in Nanoporous Latex Coatings of Rhodopseudomonas palustris CGA009 during Dry Storage at Ambient Temperatures. Microbial Biotechnology 6:515–525.

Stallings KD, Kitchener RL, Hentz NG. 2014. A High-Temperature, High Throughput Method for Monitoring Residual Formaldehyde in Vaccine Formulations. Journal of Laboratory Automation 19:275–284.

Susanti RF, Han YS, Kim J, Lee YH, Carbonell RG. 2013. A New Strategy for Ultralow Biofouling Membranes: Uniform and Ultrathin Hydrophilic Coatings Using Liquid Carbon Dioxide. Journal of Membrane Science 440:88–97.

Wang E, Allen A, Campos A, Killens Cade R, Dean L, Dvora M, Immer JG, Mixson S, Srirangan S, Sauer S, et al. 2013. ASI: Dunaliella Marine Microalgae to Drop-In Replacement Liquid Transportation Fuel. Environmental Progress and Sustainable Energy 32:916–925.

Wilson AR, Sun K, Chi M, White R, LeBeau JM, Lamb HH, Wiley BJ. 2013. From Core-Shell to Alloys: The Preparation and Characterization of Solution-Synthesized AuPd Nanoparticle Catalysts. Journal of Physical Chemistry C 117:7489–7496.

Witcher M, Carbonell RG, Odum J, Bigelow P, Lewis P, Zivitz M. 2013. Facility of the Future: Next Generation Biomanufacturing Forum, Part I. Pharmaceutical Engineering 30:1–7.

Witcher M, Carbonell RG, Odum J, Bigelow P, Lewis P, Zivitz M. 2013. Facility of the Future: Next Generation Biomanufacturing Forum, Part II. Pharmaceutical Engineering 30:1–7.

Zhang Y, Carbonell RG, Rojas OJ. 2013. Bioactive Cellulose Nanofibrils for Specific Human IgG Binding. Biomacromolecules 14:4161–4168.

Zhang Y, Islam N, Carbonell RG, Rojas OJ. 2013. Specific Binding of Immunoglobulin G with Bioactive Short Peptides Supported on Antifouling Copolymer Layers for Detection in Quartz Crystal Microgravimetry and Surface Plasmon Resonance. Analytical Chemistry 85:1106–1113.

Zhang Y, Islam N, Carbonell RG, Rojas OJ. 2013. Specificity and Regenerability of Short Peptide Ligands Supported on Polymer Layers for Immunoglobulin G Binding and Detection. ACS Applied Materials and Interfaces 5:8030–8037.


Bonino CA, Efimenko K, Jeong SI, Krebs MD, Alsberg E, Khan SA. 2012. Three-Dimensional Electrospun Alginate Nanofiber Mats via Tailored Charge Repulsions. Small 8:1928–1936.

Datta P, Efimenko K, Genzer J. 2012. The Effect of Confinement on Thermal Frontal Polymerization. Polymer Chemistry 12:3243–3246.

Flickinger M ed. 2012. Advancing Coatings with Biotechnology. In: Coatings.

Gera N, Hill AB, White DP, Carbonell RG, Rao BM. 2012. Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold. PLoS ONE 7.

Gosse JL, Chinn MS, Grunden AM, Flickinger MC. 2012. A Versatile Method for the Preparation of Hydrated Microbial-latex Biocatalytic Coatings for Headspace Gas Absorption and Gas Evolution. Journal of Industrial Microbiology and Biotechnology 39:1269–1278.

Heldt CL, Gurgel PV, Jaykus L-A, Carbonell RG. 2012. Porcine Parvovirus Removal Using Trimer and Biased Hexamer Peptides. Biotechnology Journal 7:558–565.

Jenkins JS, Flickinger MC, Velev OD. 2012. Deposition of Composite Coatings from Particle-Particle and Particle-Yeast Cell Blends by Convective-Sedimentation Assembly. Journal of Colloid and Interface Science 380:192–200.

Katharios-Lanwermeyer S, Rakic-Martinez M, Elhanafi D, Ratani S, Tiedje JM, Kathariou S. 2012. Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae. Applied and Environmental Microbiology 78:7549–7556.

Menegatti S, Naik AD, Gurgel PV, Carbonell RG. 2012. Alkaline-Stable Peptide Ligand Affinity Adsorbents for the Purification of Biomolecules. Journal of Chromatography A 1245:55–64.

Menegatti S, Naik AD, Gurgel PV, Carbonell RG. 2012. Purification of Polyclonal Antibodies from Cohn Fraction II + III, Skim Milk, and Whey by Affinity Chromatography Using a Hexamer Peptide Ligand. Journal of Separation Science 35:3139–3148.

Mota M, Flickinger MC. 2012. Modeling the Influence of Slurry Concentration on Saccharomyces cerevisiae Cake Porosity and Resistance During Microfiltration. Biotechnology Progress 28:1534–1541.

Naik AD, Menegatti S, Reese HR, Gurgel PV, Carbonell RG. 2012. Process for Purification of Monoclonal Antibody Expressed in Transgenic Lemna Plant Extract Using Dextran-Coated Charcoal and a Hexamer Peptide Affinity Resin. Journal of Chromatography A 1260:61–66.