BEC 440/BEC 595-001
Expression Systems in Biomanufacturing

Instructor

Dr. Gisele Passador-Gurgel

Credits

3.0

Schedule

View course schedule

Course Description

Introduction to various expression systems, their advantages and disadvantages. It includes basic techniques in DNA cloning, cell transformation and optimization of protein expression, selection, archiving and characterization of production line. The lab portion of the course provides students with practical experience in DNA cloning and protein expression techniques in diverse expression systems.

Selected Lecture Topics

  • Choice of expression system - bacterial, yeast, algae, fungal, insect cells, and mammalian cells
  • The Genetic Construct - DNA regulatory elements, coding region, selectable markers, and accessory genes
  • Protein expression - folding and processing
  • Methods of transformation and selection of cell lines
  • Establishment of the Master Cell Bank - description, documentation and SOPs for establishing the Master Bank

Selected Lab Topics

  • Preparation of plasmid DNA
  • Transformation of bacteria, yeast and insect cell line with plasmid DNA
  • Screening colonies for transformants
  • Isolation and characterization of DNA from transformants
  • Extraction and quantification of recombinant protein
  • Selection of expression system with highest recombinant protein yield and activity

Prerequisites & Requirements

Prerequisites

BEC 363 or MB 351 and MB 352

Text Requirements

All lectures, lab modules, and reading assignments will be available on the Moodle site for the course or will be provided in hard copy by the instructor.